Smp 099770

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GeneDB ID: smp_099770

Protein Name: SmTSP7

Full Length clone: Yes


Full Length clone sequence

ATGAAGCTCAAGCTGACGCAACAATTTTGGTTCTATGTCTTCATTGTATT AAATTGTTTCCTTGGAGTAACCAGCTTCATTTTATTTGTATTAAGTATTA AAGCACAAGATCATTTATTTCAATATAAATTAATTATACAATATACAATA CCAGCTATCTATCCAACTGGAATATTCACCGGATGTCTTGGTTTAGTTAC TACATGTTTAGGATTTCTTGGAATATGGAGAAAAATCAATATTTTTTATA TTTTACATGTTATTTGTTTGACCATAGCAACAATAATTAATCTATGTATA GCTGTTGTATCGGTTATCATTGATGATCAATTTTTTATTAATGCTAAAGA AGCCTTGAATACTACTATTAAATATTATTATGAGAAGAATGAATATGGAG ATGAATTTGATAAATTACATATGACATTCTTCTGTTGTGGAGTTAATTCA TATGCTGATTTTAGAAAAGCTAAATTATTGATACCATATTCGTGTAGAGT TGGTCAGTTTGTTTATGCAAGAGGTTGCTATGATGAATTAAGTGATTTCG TACAATATTACATAACTCTATTAACATCCATTTGTTTTATTACATCATTT ATTTATATTGTATTCATTGGTGTATCAATTCTGAATCTTCATAAATCAAT AAAGGGAAAGAATCCATCTTCTTCATGA


Primers used

smp_099770 FL F: AAACAACAATGAAGCTCAAG (partially 5' UTR)

smp_099770 FL R: GAATGTAGTTAAGTTGAATTCTCA (completely in 3' UTR)

Vector

pCR-Blunt (Zeroblunt - Invitrogen) Kanamycin resistance

Glycerol Stock

Box 1 R1C3

Microarray Lifecycle Profile

Smp 099770.jpg

50mer - Contig6455

Data obtained from Fitzpatrick et al., 2009

Protein Expression

Expression Vector - modified pET30a

Sequence modified/truncated - Yes (only extracellular loop 2 expressed)

Purification tag - 6XHis (c-terminal only)

Expressed protein sequence

MDDQFFINAKEALNTTLKYYYEKNEYGDEFDKLHMTFFCCGVNSYADFRKAKLLIPYSCR VGQFVYARGCYDELSDFVQLEHHHHHH

Expressed protein MW/PI - 10.4kDa / 5.91

Literature